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Human ENPP-2/Autotaxin Protein 4789

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Active: Yes (catalytic)
  • Amino Acid Range: Asp49-Ile863
SKU: 4789parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

NP_001035181

express system

HEK293

product tag

N-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.

molecular weight

The protein has a predicted MW of 94.8 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human ENPP-2/Autotaxin Protein 4789

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2) also known as Autotaxin, is a secreted lysophospholipase D, which hydrolyzes lysophosphatidylcholine (LPC) into Lysophosphatidic acid (LPA). ENPP2 is an essential protein for normal development and its altered expression is associated with various human diseases.
Protein names
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2) (EC 3.1.4.39) (Autotaxin) (Extracellular lysophospholipase D) (LysoPLD)
Gene names
Enpp2,Enpp2 Npps2 Pdnp2
Protein family
Nucleotide pyrophosphatase/phosphodiesterase family
Mass
10090Da
Function
Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:17208043, PubMed:27780639, PubMed:28414242). Major substrate is lysophosphatidylcholine (PubMed:17208043, PubMed:27780639). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:18175805). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition (By similarity). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (By similarity). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (By similarity).
Subellular location
Secreted .
Tissues
Expressed in brain and adipose tissue.
Post-translational modification
N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. Secretion requires simultaneous glycosylation on Asn-53 and Asn-410, while probable glycosylation of Asn-410 has a preferential role on lysoPLD activity. Not O-glycosylated.; The interdomain disulfide bond between Cys-413 and Cys-805 is essential for catalytic activity.
Target Relevance information above includes information from UniProt accession: Q9R1E6
The UniProt Consortium

Data

Activity assay with Human ENPP-2/Autotaxin Protein
Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is > 10000 pmol/min/µg, as measured under the described conditions.
HPLC of Human ENPP-2/Autotaxin Protein
The purity of Human ENPP-2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human ENPP-2/Autotaxin Protein
Human ENPP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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