Rat GDF15 Protein 2869

$450.00 – $1,500.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Ser189-Ala303

SKU: 2869parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9Z0J6
express system	E.coli
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE
background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
molecular weight	The protein has a predicted MW of 13.68 kDa. The protein migrates to 15-17 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Rat GDF15 Protein 2869
protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Protein names
Growth/differentiation factor 15 (GDF-15)
Gene names
Gdf15,Gdf15 Sbf
Protein family
TGF-beta family
Mass
10116Da
Function
Hormone produced in response to various stresses to confer information about those stresses to the brain, and trigger an aversive response, characterized by nausea and/or loss of appetite (PubMed:28846099, PubMed:28953886, PubMed:31928886, PubMed:34187898, PubMed:37060902). The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future (By similarity). Acts by binding to its receptor, GFRAL, activating GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed:28846099, PubMed:28953886, PubMed:37060902). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes responses to stressful conditions (PubMed:37060902). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (By similarity). Contributes to the effect of metformin, an anti-diabetic drug, on appetite reduction and weight loss: produced in the kidney in response to metformin treatment, thereby activating the GDF15-GFRAL response, leading to reduced appetite and weight (PubMed:37060902). Required for avoidance behavior in response to food allergens: induced downstream of mast cell activation to promote aversion and minimize harmful effects of exposure to noxious substances (By similarity). Produced in response to anticancer drugs, such as camptothecin or cisplatin, promoting nausea and contributing to malnutrition (By similarity). Overproduced in many cancers, promoting anorexia in cancer (cachexia) (By similarity). Responsible for the risk of nausea during pregnancy: high levels of GDF15 during pregnancy, mostly originating from embryos, are associated with increased nausea (By similarity). Maternal sensitivity to nausea is probably determined by pre-pregnancy exposure to GDF15, females with naturally high level of GDF15 being less susceptible to nausea than female rats with low levels of GDF15 before pregnancy (By similarity). Promotes metabolic adaptation in response to systemic inflammation caused by bacterial and viral infections in order to promote tissue tolerance and prevent tissue damage (By similarity). Required for tissue tolerance in response to myocardial infarction by acting as an inhibitor of leukocyte integring activation, thereby protecting against cardiac rupture (By similarity). Inhibits growth hormone signaling on hepatocytes (By similarity).
Subellular location
Secreted. Note=Secreted in the plasma.
Tissues
Detected in plasma (at protein level).
Structure
Homodimer; disulfide-linked (By similarity). Interacts with GFRAL and RET; ligand of GFRAL, which mediates GDF15 internalization and cellular signaling through interaction with RET via the formation of a 2:2:2 ternary complex composed of GDF15, GFRAL and RET (By similarity).
Target Relevance information above includes information from UniProt accession: Q9Z0J6
The UniProt Consortium

Immobilized Rat GDF-15, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test).

Immobilized Rat GDF15, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.95µg/ml determined by ELISA.

Rat GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.