ELISA with Cynomolgus TPBG/5T4 Protein 3066

Cynomolgus TPBG/5T4 Protein 3066

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Thr35-Ser355

SKU: 3066parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q4R8Y9
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
molecular weight	The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus TPBG/5T4 Protein 3066

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Protein names Trophoblast glycoprotein (Wnt-activated inhibitory factor 1) (WAIF1)
Gene names TPBG,TPBG QtsA-11109
Mass 9541Da
Function May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Post-translational modification Highly glycosylated.
Domain Th
Target Relevance information above includes information from UniProt accession: Q4R8Y9
The UniProt Consortium

Immobilized Cynomolgus TPBG, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.

The purity of Cynomolgus TPBG is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus TPBG/5T4 Protein

Cynomolgus TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. What is the source of the Cynomolgus TPBG/5T4 protein offered by Benchmark Antibodies?
- The Cynomolgus TPBG/5T4 protein is expressed and purified from HEK293 cells to ensure high purity and biological activity.

2. How should I store the Cynomolgus TPBG/5T4 protein for optimal stability?
- We recommend storing the protein at -80°C upon arrival to maintain its integrity. Avoid repeated freeze-thaw cycles to preserve its activity.

3. Is the Cynomolgus TPBG/5T4 protein compatible with a specific assay or application?
- This protein is suitable for various applications, including ELISA, Western blotting, and functional studies related to cancer research and immunotherapy.

4. Can the Cynomolgus TPBG/5T4 protein be used in cell culture experiments?
- Yes, the protein can be used in cell culture experiments to study its role in cell signaling, proliferation, and interaction with antibodies or potential therapeutics.

5. Does Benchmark Antibodies provide technical support or guidance on using the Cynomolgus TPBG/5T4 protein in specific experiments?
- Yes, our team of experts is available to provide technical support and assistance regarding the use of the Cynomolgus TPBG/5T4 protein in your research projects. Feel free to reach out with any questions or concerns.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.