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Rat ANG-1 (Angiopoietin-1) ELISA Kit 60042

$570.00

Summary

  • ELISA kit for research use (RUO)
  • Reactivity: ANG1
  • Suitable for: ELISA
  • Time:3 hours
  • Sample types:Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
SKU: 60042 Category: Tags: , , ,
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

ANG1

species reactivity

Rat

applications

Double Antibody, Sandwich ELISA

assay type

direct & quantitative

available size

96 tests

sensitivity

0.094ng/ml

range

0.156-10ng/ml

Rat ANG-1 (Angiopoietin-1) ELISA Kit

kit
Assay type
sandwich (sELISA)
Research area
biologic
Sample type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Components
Break apart microtiter test strips each coated single wells 8 x 12 (96 Total)
Lyophilized Standard2 x vial
Biotin-labeled Antibody(Concentrated, 100X)120 uL
HRP-Streptavidin Conjugate(Concentrated, 100X)120 uL
Washing solution concentrate (25X)30 mL
Sample Dilution buffer20 mL
Antibody Dilution buffer10 mL
Streptavidin Dilution buffer10 mL
Stopping solution10 mL
TMB Substrate (ready-to-use)10 mL
Plate seals3
Storage
Store at 2-8°C.
target relevance
ANG1
ANG1 biologic drug binds Rattus norvegicus ANGPT1 Angiopoietin-1

Data

Standard Curve

X axis: log | Y axis: linear
Standard STD [ng/mL] OD1 OD2 Average OD Corrected OD
Blank #8 0 0.14 0.15 0.15 0
Standard #7 0.16 0.21 0.21 0.21 0.06
Standard #6 0.31 0.35 0.36 0.36 0.21
Standard #5 0.63 0.47 0.49 0.48 0.34
Standard #4 1.25 0.82 0.84 0.83 0.69
Standard #3 2.5 1.26 1.29 1.27 1.13
Standard #2 5 1.73 1.78 1.75 1.61
Standard #1 10 2.25 2.31 2.28 2.13

Precision

Sample n Mean Standard Deviation CV (%)
Intra Sample 1 20 0.32 0.02 5.23
Intra Sample 2 20 1.23 0.06 4.88
Intra Sample 3 20 4.95 0.25 4.98
Inter Sample 1 20 0.32 0.02 6.2
Inter Sample 2 20 1.24 0.07 5.72
Inter Sample 3 20 4.95 0.27 5.54

Recovery

Matrix Recovery Range (%) Average (%)
Serum(n=5) 88-104 96
EDTA Plasma(n=5) 86-101 94
HeparinPlasma(n=5) 85-98 93

Stability

37°C 1 Month (%) 2–8°C 6 Months (%) 2–8°C 12 Months (%)
80 95-100 85-98

Linearity

Sample 1:2 Dilution (%) 1:4 Dilution (%) 1:8 Dilution (%)
Serum(n=5) 90-105% 87-96% 87-95%
EDTAPlasma(n=5) 85-104% 91-100% 80-100%
Heparin Plasma(n=5) 85-102% 83-97% 81-89%

FAQ & Publications

Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

Relevant to this product
View Protocol  |  View PDF

Documents

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