Mouse VCAM-1/CD106 Protein 2435

$315.00 – $1,050.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Phe25-Glu698

SKU: 2435parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_035823.3
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.
molecular weight	The protein has a predicted MW of 75.48 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse VCAM-1/CD106 Protein 2435

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.
Protein names Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (INCAM-100) (CD antigen CD106) [Cleaved into: Soluble Vascular Cell Adhesion Molecule-1]
Mass 81276Da
Function Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567). {ECO:0000269\|PubMed:10209034, ECO:0000269\|PubMed:22970700, ECO:0000269\|PubMed:31310649, ECO:0000269\|PubMed:35210567}.
Subellular location [Vascular cell adhesion protein 1]: Cell membrane {ECO:0000305\|PubMed:12878595}; Single-pass type I membrane protein.; [Soluble Vascular Cell Adhesion Molecule-1]: Secreted {ECO:0000269\|PubMed:12878595, ECO:0000269\|PubMed:36127634}.
Tissues Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Post-translational modification Cleaved by the metalloproteinase ADAM17 to generate the soluble form. {ECO:0000269\|PubMed:12878595}.; Sialoglycoprotein.; Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation. {ECO:0000269\|PubMed:31310649}.
Domain Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell ad
Target Relevance information above includes information from UniProt accession: P19320
The UniProt Consortium

Data

Cell based assay with Mouse VCAM-1/CD106 Protein

Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to Mouse VCAM-1 coated plates (10 µg/ml with 100 µl/Well) , approximately 70 % - 80 % cells will adhere after 1 hour incubation at room temperature.

The purity of Mouse VCAM-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse VCAM-1/CD106 Protein

Mouse VCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.