Mouse TNFRSF11A/Rank Protein 2989

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val31-Ser214

SKU: 2989parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O35305
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.
molecular weight	The protein has a predicted MW of 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse TNFRSF11A/Rank Protein 2989

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.
Protein names Tumor necrosis factor receptor superfamily member 11A (Osteoclast differentiation factor receptor) (ODFR) (Receptor activator of NF-KB) (CD antigen CD265)
Gene names Tnfrsf11a,Tnfrsf11a Rank
Mass 10090Da
Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed:20483727, PubMed:23478294, PubMed:9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (PubMed:23478294). Involved in the regulation of interactions between T-cells and dendritic cells (PubMed:9367155).
Catalytic activity BINDING 134; /ligand="Na(+)"; /ligand_id="ChEBI:CHEBI:29101"; BINDING 135; /ligand="Na(+)"; /ligand_id="ChEBI:CHEBI:29101"; BINDING 138; /ligand="Na(+)"; /ligand_id="ChEBI:CHEBI:29101"; BINDING 161; /ligand="Na(+)"; /ligand_id="ChEBI:CHEBI:29101"; BINDING 163; /ligand="Na(+)"; /ligand_id="ChEBI:CHEBI:29101"
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft .
Tissues Ubiquitous expression with high levels in trabecular bone, thymus, small intestine, lung, brain and kidney. Weakly expressed in spleen and bone marrow.
Structure Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer (PubMed:20483727, PubMed:23039992). Part of a complex composed of EEIG1, TNFRSF11A/RANK, PLCG2, GAB2, TEC and BTK; complex formation increases in the presence of TNFSF11/RANKL (PubMed:23478294). Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 (By similarity). Interacts (via cytoplasmic domain) with GAB2 (By similarity). Interacts (via cytoplasmic domain); with EEIG1 (via N-terminus); when in the presence of TNFSF11/RANKL (PubMed:23478294).
Target Relevance information above includes information from UniProt accession: O35305
The UniProt Consortium

Immobilized Mouse RANKL, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 8.5ng/ml determined by ELISA.

The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse TNFRSF11A/Rank Protein

Mouse TNFRSF11A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.