Mouse MEPE Protein 3987

$270.00 – $900.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Ala25-Asp441

SKU: 3987parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8K4L6
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.
molecular weight	The protein has a predicted MW of 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse MEPE Protein 3987

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.
Protein names Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) (OF45) (Osteoregulin)
Gene names Mepe,Mepe
Protein family PF07175/osteoregulin family
Mass 46872Da
Function FUNCTION: Regulates renal phosphate and uric acid excretion (PubMed:26051469). Regulates bone mineralization by osteoblasts and cartilage mineralization by chondrocytes (PubMed:11414762, PubMed:12421822, PubMed:15843468, PubMed:22766095). Regulates the mineralization of the extracellular matrix of the craniofacial complex, such as teeth, bone and cartilage (PubMed:26927967). Increases dental pulp stem cell proliferation (By similarity). {ECO:0000250\|UniProtKB:Q9NQ76, ECO:0000269\|PubMed:11414762, ECO:0000269\|PubMed:12421822, ECO:0000269\|PubMed:15843468, ECO:0000269\|PubMed:22766095, ECO:0000269\|PubMed:26051469, ECO:0000269\|PubMed:26927967}.
Subellular location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000269\|PubMed:12421822, ECO:0000269\|PubMed:18597632, ECO:0000269\|PubMed:26051469}. Secreted {ECO:0000250\|UniProtKB:Q9NQ76}.
Tissues TISSUE SPECIFICITY: Expressed in osteocytes (at protein level) (PubMed:12421822, PubMed:15221418). Expressed by chondrocytes, specifically in the hypertrophic zone of the bone growth plate (at protein level) (PubMed:22766095). Expressed in osteoblasts in bone (at protein level) (PubMed:11414762, PubMed:15221418, PubMed:18597632). Expressed by osteoblasts within the metaphysis (at protein level) (PubMed:15221418, PubMed:22766095). Expressed at low levels in white fat, brown fat, testes, brain and aorta (PubMed:12421822). Expressed in the craniofacial complex (at protein level) (PubMed:26927967). Expressed in odontoblasts, ameloblasts and in predentin during tooth development (at protein level) (PubMed:22042093). Expressed in the kidney (at protein level) (PubMed:26051469). Expressed in osteocytes in mandibular condylar cartilage and tibial cartilage (at protein level) (PubMed:26428891). Expressed in salivary glands (PubMed:15329369). {ECO:0000269\|PubMed:11414762, ECO:0000269\|PubMed:12421822, ECO:0000269\|PubMed:15329369, ECO:0000269\|PubMed:18597632, ECO:0000269\|PubMed:22042093, ECO:0000269\|PubMed:22766095, ECO:0000269\|PubMed:26051469, ECO:0000269\|PubMed:26428891, ECO:0000269\|PubMed:26927967}.
Structure SUBUNIT: Interacts (via ASARM motif) with PHEX; the interaction is zinc-dependent. {ECO:0000250\|UniProtKB:Q9NQ76}.
Post-translational modification PTM: Phosphorylated on serine residues in the ASARM motif; the phosphorylation is important for the inhibition of bone mineralization. {ECO:0000269\|PubMed:15843468, ECO:0000269\|PubMed:18597632, ECO:0000269\|PubMed:22766095}.; PTM: Cleaved by CTSB/cathepsin B; the cleavage is blocked by metalloprotease PHEX. {ECO:0000250\|UniProtKB:Q9NQ76}.
Domain DOMAIN: The acidic serine aspartate-rich MEPE-associated (ASARM) motif is sufficient when phosphorylated to inhibit bone mineralization by osteoblasts and cartilage mineralization by chondrocytes by binding hydroxyapatite crystals during the mineralization stage (PubMed:15843468, PubMed:22766095, PubMed:26051469). It can also inhibit dentin mineralization (By similarity). {ECO:0000250\|UniProtKB:Q9NQ76, ECO:0000269\|PubMed:15843468, ECO:0000269\|PubMed:22766095, ECO:0000269\|PubMed:26051469}.; DOMAIN: The dentonin region is sufficient to promote dental pulp stem cell proliferation. It can also stimulate bone formation, osteoblast differentiation, and activate integrin signaling pathways. {ECO:0000250\|UniProtKB:Q9NQ76}.
Target Relevance information above includes information from UniProt accession: Q8K4L6
The UniProt Consortium

Data

Mouse MEPE on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.