Mouse LILRB4/CD85k/ILT3 Protein 3953

$300.00 – $1,000.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Gly24-Lys238

SKU: 3953parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q64281
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	LILRB4, also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
molecular weight	The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse LILRB4/CD85k/ILT3 Protein 3953

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
LILRB4,also known as CD85k and LIR-5, ILT3,Â is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
Protein names Leukocyte immunoglobulin-like receptor subfamily B member 4A (Mast cell surface glycoprotein Gp49B) (CD antigen CD85k)
Gene names Lilrb4a,Lilrb4a Gp49b
Mass 10090Da
Function Inhibitory receptor involved in the down-regulation of the immune response (PubMed:10026201, PubMed:24935931). Receptor for FN1 (PubMed:34089617). Receptor for integrin ITGAV/ITGB3 (PubMed:11323698). Inhibits IgE-mediated mast cell activation, at least in part through interaction with ITGAV/ITGB3 (PubMed:10026201, PubMed:11323698, PubMed:11457897, PubMed:8855262). Also inhibits KITLG/SCF-mediated mast cell activation (PubMed:12884301). Through interaction with ITGAV/ITGB3, inhibits antibody production by memory and marginal zone B cells, probably by suppressing their differentiation into plasma cells (PubMed:24935931). Inhibits IFNG production by CD8 T cells, CD4 T cells and natural killer cells (PubMed:12682239). Inhibits antigen presentation by dendritic cells to T cells, preventing T cell activation (PubMed:18792399). Inhibits lipopolysaccharide-mediated neutrophil-dependent vascular injury (PubMed:14557414). Suppresses the allergic inflammatory response by inhibiting infiltration of neutrophils and eosinophils and preventing mast cell degranulation (PubMed:17761953). Inhibits lysis by natural killer cells (PubMed:8977169).
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed on mast cells and natural killer cells (at protein level) (PubMed:11457897, PubMed:8977169, PubMed:8977170). Expressed on neutrophils (at protein level) (PubMed:14557414). Expressed on eosinophils (at protein level) (PubMed:17761953). Expressed on dendritic cells (at protein level) (PubMed:18792399). Expressed on memory and marginal zone B cells (at protein level) (PubMed:24935931). Expressed on CD8 T cells (at protein level) (PubMed:12682239). Expressed in the uterus of pregnant mice where it is detected at day 4.0 of pregnancy with levels dropping at day 4.5 (PubMed:9338594). Highly expressed in the luminal epithelium of uterine endometrium with lower levels in the glandular epithelium (PubMed:9338594).
Structure Interacts (when tyrosine phosphorylated) with SH2 domain-containing phosphatases PTPN6/SHP-1 and PTPN11/SHP-2; interaction with PTPN6 enhances inhibition of mast cell activation.
Post-translational modification Tyrosine phosphorylated.
Domain Co
Target Relevance information above includes information from UniProt accession: Q64281
The UniProt Consortium

SPR with Mouse LILRB4/CD85k/ILT3 Protein

Mouse LILRB4, His Tag immobilized on CM5 Chip can bind Mouse APOE, His Tag with an affinity constant of 5.08 nM as determined in SPR assay (Biacore T200).

The purity of Mouse LILRB4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse LILRB4/CD85k/ILT3 Protein

Mouse LILRB4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.