Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
accession | P43431(IL2A)&P43432(IL2B) |
express system | HEK293 |
product tag | C-His |
purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
background | Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
molecular weight | The protein has a predicted MW of 22.80 kDa(IL-12A)&35.79 kDa(IL-12B). Due to glycosylation, the protein migrates to 25-30 kDa(IL-12A)&45-55 kDa(IL-12B) based on Tris-Bis PAGE result. |
available size | 100 µg, 500 µg |
endotoxin | Less than 1EU per μg by the LAL method. |
Mouse IL-12 Protein 2981
$450.00 – $1,500.00
Summary
- Expression: HEK293
- Active: Yes (cell based assay)
- Amino Acid Range: Arg23-Ala215(IL-12A) & Met23-Ser335(IL-12B)
Mouse IL-12 Protein 2981
protein |
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Size and concentration 100, 500µg and lyophilized |
Form Lyophilized |
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Storage buffer Shipped at ambient temperature. |
Purity > 95% as determined by Tris-Bis PAGE |
target relevance |
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Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
Protein names Interleukin-12 subunit alpha (IL-12A) (Cytotoxic lymphocyte maturation factor 35 kDa subunit) (CLMF p35) (IL-12 subunit p35) |
Gene names Il12a,Il12a |
Protein family IL-6 superfamily |
Mass 24179Da |
Function Heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to form the IL-35 cytokine. IL-12 is primarily produced by professional antigen-presenting cells (APCs) such as B-cells and dendritic cells (DCs) as well as macrophages and granulocytes and regulates T-cell and natural killer-cell responses, induces the production of interferon-gamma (IFN-gamma), favors the differentiation of T-helper 1 (Th1) cells and is an important link between innate resistance and adaptive immunity (PubMed:8766560). Mechanistically, exerts its biological effects through a receptor composed of IL12R1 and IL12R2 subunits. Binding to the receptor results in the rapid tyrosine phosphorylation of a number of cellular substrates including the JAK family kinases TYK2 and JAK2. In turn, recruited STAT4 gets phosphorylated and translocates to the nucleus where it regulates cytokine/growth factor responsive genes (By similarity). As part of IL-35, plays essential roles in maintaining the immune homeostasis of the liver microenvironment and functions also as an immune-suppressive cytokine (PubMed:18033300, PubMed:30197594). Mediates biological events through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers. Signaling requires the transcription factors STAT1 and STAT4, which form a unique heterodimer that binds to distinct DNA sites (By similarity). |
Subellular location Secreted . |
Structure Heterodimer with IL12B; disulfide-linked (PubMed:1350290). This heterodimer is known as interleukin IL-12 (PubMed:1350290). Heterodimer with EBI3/IL27B; not disulfide-linked (By similarity). This heterodimer is known as interleukin IL-35 (By similarity). Interacts with NBR1; this interaction promotes IL-12 secretion (PubMed:34374750). |
Target Relevance information above includes information from UniProt accession: P43431 |
The UniProt Consortium |
Publications
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