Mouse Fc gamma RI/CD64 Protein 3445

$240.00 – $800.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Glu25-Pro297

SKU: 3445parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P26151
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
molecular weight	The protein has a predicted MW of 31.5 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Mouse Fc gamma RI/CD64 Protein 3445

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Protein names High affinity immunoglobulin gamma Fc receptor I (IgG Fc receptor I) (Fc-gamma RI) (FcRI) (CD antigen CD64)
Gene names Fcgr1,Fcgr1 Fcg1
Protein family Immunoglobulin superfamily, FCGR1 family
Mass 44888Da
Function High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Subellular location Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.
Tissues Macrophage-specific.
Structure Interacts with FCERG1; forms a functional signaling complex (By similarity). Interacts with FLNA; prevents FCGR1A degradation (By similarity). Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN (By similarity).
Post-translational modification N-glycosylated.; Phosphorylated on serine residues.
Target Relevance information above includes information from UniProt accession: P26151
The UniProt Consortium

Data

Trastuzumab captured on CM5 Chip via Protein A can bind Mouse Fc gamma RI, His Tag with an affinity constant of 87.0 nM as determined in SPR assay (Biacore T200).

The purity of Mouse Fc gamma RI is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Fc gamma RI/CD64 Protein

Mouse Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.