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Mouse CD73/NT5E Protein 4493

$270.00$900.00

Summary

  • Expression: HEK293
  • Active: Yes (catalytic)
  • Amino Acid Range: Trp29-Lys549
SKU: 4493parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q61503

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.

molecular weight

The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Mouse CD73/NT5E Protein 4493

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside‑5'‑monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury.
Protein names
5'-nucleotidase (5'-NT) (EC 3.1.3.35) (EC 3.1.3.5) (EC 3.1.3.89) (EC 3.1.3.91) (EC 3.1.3.99) (5'-deoxynucleotidase) (Ecto-5'-nucleotidase) (IMP-specific 5'-nucleotidase) (Thymidylate 5'-phosphatase) (CD antigen CD73)
Gene names
Nt5e,Nt5e Nt5 Nte
Protein family
5'-nucleotidase family
Mass
10090Da
Function
FUNCTION: Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:8224905). Hydrolyzes IMP (PubMed:8224905). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (By similarity). Although AMP is the preferred substrate can also hydrolyze UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (By similarity). {ECO:0000250|UniProtKB:P21589, ECO:0000269|PubMed:8224905}.
Catalytic activity
BINDING 38; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 38; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 40; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 87; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 87; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 119; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 222; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 245; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 356; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 356; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 392; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 392; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 397; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 397; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 419; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 419; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 502; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 502; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 508; /ligand="AMP"; /ligand_id="ChEBI:CHEBI:456215"; /evidence="ECO:0000250|UniProtKB:P21589"; BINDING 508; /ligand="IMP"; /ligand_id="ChEBI:CHEBI:58053"; /evidence="ECO:0000250|UniProtKB:P21589"
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P21589}; Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:P21589}.
Tissues
TISSUE SPECIFICITY: Expressed at high levels in the placenta, kidney, lung and stomach and at lower levels in the thymus, spleen, skeletal muscle and esophagus. {ECO:0000269|PubMed:8224905}.
Structure
SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P21589}.
Target Relevance information above includes information from UniProt accession : Q61503
The UniProt Consortium

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