Mouse CD14 Protein 2871

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Ser16-Pro345

SKU: 2871parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_033971
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD14 is a glycosylphosphatidylinositol (GPI)-anchored receptor known to serve as a co-receptor for several Toll-like Receptors (TLRs) both at the cell surface and in the endosomal compartment. CD14 can be expressed by cells of both hematopoietic and non-hematopoietic origin as a cell membrane or secreted protein.
molecular weight	The protein has a predicted MW of 38.19 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse CD14 Protein 2871

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD14 is a glycosylphosphatidylinositol (GPI)-anchored receptor known to serve as a co-receptor for several Toll-like Receptors (TLRs) both at the cell surface and in the endosomal compartment. CD14 can be expressed by cells of both hematopoietic and non-hematopoietic origin as a cell membrane or secreted protein.
Protein names Monocyte differentiation antigen CD14 (My23 antigen) (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form]
Mass 40076Da
Function Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187). {ECO:0000269\|PubMed:16880211, ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:20133493, ECO:0000269\|PubMed:22265692, ECO:0000269\|PubMed:23264655, ECO:0000269\|PubMed:23880187, ECO:0000269\|PubMed:8612135}.
Subellular location Cell membrane {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:3385210}; Lipid-anchor, GPI-anchor {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:3385210}. Secreted {ECO:0000269\|PubMed:25497142, ECO:0000269\|PubMed:2779588}. Membrane raft {ECO:0000269\|PubMed:16880211}. Golgi apparatus {ECO:0000269\|PubMed:16880211}. Note=Secreted forms may arise by cleavage of the GPI anchor. {ECO:0000269\|PubMed:2462937, ECO:0000269\|PubMed:2779588, ECO:0000269\|PubMed:3385210}.
Tissues Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. {ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:25497142}.
Structure Interacts with LPS-bound LPB (PubMed:1698311, PubMed:23264655). Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4 (PubMed:11274165). Interacts with LPAR1 (By similarity). Interacts with the TLR2:TLR6 or TLR2:TLR1 heterodimers; upon interaction with ligands such as diacylated lipopeptides and triacylated lipopeptides, respectively (PubMed:16880211). Interacts with MYO18A (PubMed:25965346). Interacts with FSTL1 (PubMed:22265692). {ECO:0000250\|UniProtKB:P10810, ECO:0000269\|PubMed:11274165, ECO:0000269\|PubMed:16880211, ECO:0000269\|PubMed:1698311, ECO:0000269\|PubMed:22265692, ECO:0000269\|PubMed:23264655, ECO:0000269\|PubMed:25965346}.
Post-translational modification N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. {ECO:0000269\|PubMed:16335952, ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:19838169}.
Domain The C-terminal leucine-rich repeat (LRR) region is required for responses to smooth LPS. {EC
Target Relevance information above includes information from UniProt accession: P08571
The UniProt Consortium

Data

The purity of Mouse CD14 is greater than 95% as determined by SEC-HPLC.

Mouse CD14 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.