Mouse CA2/Carbonic anhydrase II Protein 2318

$270.00 – $900.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Ser2-Lys260

SKU: 2318parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P00920
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and CO2 to bicarbonate and a proton. CA II is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular pH control, and vascular regulation.
molecular weight	The protein has a predicted MW of 29.99 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse CA2/Carbonic anhydrase II Protein 2318

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Carbonic anhydrase II (CA II) is a zinc metalloenzyme that catalyzes the reversible interconversion of water and CO2 to bicarbonate and a proton. CA II is abundant in most cells, and plays a role in numerous processes including gas exchange, epithelial ion transport, respiration, extra- and intracellular pH control, and vascular regulation.
Protein names Carbonic anhydrase 2 (EC 4.2.1.1) (Carbonate dehydratase II) (Carbonic anhydrase II) (CA-II) (Cyanamide hydratase CA2) (EC 4.2.1.69)
Gene names Ca2,Ca2 Car2
Protein family Alpha-carbonic anhydrase family
Mass 10090Da
Function Catalyzes the reversible hydration of carbon dioxide (By similarity). Can also hydrate cyanamide to urea (By similarity). Involved in the regulation of fluid secretion into the anterior chamber of the eye (By similarity). Essential for bone resorption and osteoclast differentiation (By similarity). Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption (By similarity). Stimulates the chloride-bicarbonate exchange activity of SLC26A6 (By similarity).
Catalytic activity BINDING 94; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_note="catalytic"; /evidence="ECO:0000250\|UniProtKB:P00918"; BINDING 96; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_note="catalytic"; /evidence="ECO:0000250\|UniProtKB:P00918"; BINDING 119; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_note="catalytic"; /evidence="ECO:0000250\|UniProtKB:P00918"; BINDING 198..199; /ligand="substrate"; /evidence="ECO:0000250\|UniProtKB:P00918"
Subellular location Cytoplasm. Cell membrane. Note=Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells.
Structure Interacts with SLC4A4 (By similarity). Interaction with SLC4A7 regulates SLC4A7 transporter activity (By similarity). Interacts with SLC26A6 (By similarity).
Target Relevance information above includes information from UniProt accession: P00920
The UniProt Consortium

Data

Activity assay with Mouse CA2/Carbonic anhydrase II Protein

Measured by its esterase activity. The specific activity is > 100 pmol/min/µg.

HPLC of Mouse CA2/Carbonic anhydrase II Protein

The purity of Mouse CA2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse CA2/Carbonic anhydrase II Protein

Mouse CA2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.