Mouse BST1 Protein 5148

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Ala25-Ser286

SKU: 5148parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q64277
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
molecular weight	The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse BST1 Protein 5148

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
Protein names ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 (EC 3.2.2.6) (ADP-ribosyl cyclase 2) (Antigen BP3) (BP-3 alloantigen) (Bone marrow stromal antigen 1) (BST-1) (Cyclic ADP-ribose hydrolase 2) (cADPR hydrolase 2) (Leukocyte antigen 65) (Ly-65) (CD antigen CD157)
Gene names Bst1,Bst1 Bp-3 Bp3 Ly65
Protein family ADP-ribosyl cyclase family
Mass 34616Da
Function FUNCTION: Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium. May be involved in pre-B-cell growth. {ECO:0000250\|UniProtKB:Q10588}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+); Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6; Evidence={ECO:0000250\|UniProtKB:Q10588}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302; Evidence={ECO:0000250\|UniProtKB:Q10588}; CATALYTIC ACTIVITY: Reaction=NAD(+) = cyclic ADP-beta-D-ribose + nicotinamide + H(+); Xref=Rhea:RHEA:38611, ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:73672; Evidence={ECO:0000250\|UniProtKB:Q10588}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38612; Evidence={ECO:0000250\|UniProtKB:Q10588}; CATALYTIC ACTIVITY: Reaction=cyclic ADP-beta-D-ribose + H2O = ADP-D-ribose; Xref=Rhea:RHEA:38615, ChEBI:CHEBI:15377, ChEBI:CHEBI:57967, ChEBI:CHEBI:73672; Evidence={ECO:0000250\|UniProtKB:Q10588}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38616; Evidence={ECO:0000250\|UniProtKB:Q10588};
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000250\|UniProtKB:Q10588}; Lipid-anchor, GPI-anchor {ECO:0000250\|UniProtKB:Q10588}.
Tissues TISSUE SPECIFICITY: Expressed in the bone marrow, spleen and thymus in lymphoid organs, and the lung, kidney and heart in non-lymphoid organs.
Structure SUBUNIT: Homodimer. {ECO:0000250\|UniProtKB:Q10588}.
Target Relevance information above includes information from UniProt accession: Q64277
The UniProt Consortium

Data

The purity of Mouse BST1 is greater than 95% as determined by SEC-HPLC.

Mouse BST1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.