Mouse Beta Klotho Protein 3222

$330.00 – $1,100.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Phe53-Pro994

SKU: 3222parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q99N32
express system	HEK293
product tag	C-His
purity	> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
molecular weight	The protein has a predicted MW of 109.91 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse Beta Klotho Protein 3222

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
Protein names
Beta-klotho (BKL) (BetaKlotho) (Klotho beta-like protein)
Gene names
Klb,Klb Betakl
Protein family
Glycosyl hydrolase 1 family, Klotho subfamily
Mass
10090Da
Function
Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.
Catalytic activity
#N/A
Subellular location
Cell membrane ; Single-pass type III membrane protein .
Tissues
Present in liver, muscle and white adipose tissue, but not in kidney (at protein level). Expressed in liver and pancreas, and at lower levels in skin, stomach, skeletal muscle, small intestine and lung.
Structure
Interacts with FGF19; this interaction is direct. Interacts (via C-terminus) with FGF21; this interaction is direct (By similarity). Interacts with FGFR1 and FGFR4.
Domain
Co
Target Relevance information above includes information from UniProt accession: Q99N32
The UniProt Consortium

Data

Human FGFR4, hFc Tag captured on CM5 Chip via Protein A can bind Mouse Beta Klotho, His Tag with an affinity constant of 36.97 nM as determined in SPR assay (Biacore T200).

The purity of Mouse Beta Klotho Protein is greater than 90% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse Beta Klotho Protein

Mouse Beta Klotho on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.