Mouse B7-2/CD86 Protein 2142

$135.00 – $450.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val24-Lys244

SKU: 2142parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P42082
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
molecular weight	The protein has a predicted MW of 26.19 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse B7-2/CD86 Protein 2142

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
Protein names T-lymphocyte activation antigen CD86 (Activation B7-2 antigen) (Early T-cell costimulatory molecule 1) (ETC-1) (CD antigen CD86)
Gene names Cd86,Cd86
Mass 10090Da
Function Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883).
Catalytic activity #N/A
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed on activated B-cells.
Structure Homodimer. Interacts with MARCH8 (By similarity). Interacts (via cytoplasmic domain) with PHB1 and PHB2; the interactions increases after priming with CD40 (PubMed:23241883).
Post-translational modification Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
Target Relevance information above includes information from UniProt accession: P42082
The UniProt Consortium

Data

Immobilized Mouse B7-2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.

The purity of Mouse B7-2 is greater than 95% as determined by SEC-HPLC.

Mouse B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.