Immunohistochemistry (IHC) : Human yolk sac tumor stained with anti-AFP polyclonal antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells..

mouse anti-AFP monoclonal antibody (C3) 6014

$160.00 – $528.00

Antibody summary

Mouse monoclonal to AFP
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2a
Control: Fetal liver or hepatocellular carcinoma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6014parent Category: antibody Tags: AFP, anatomy, GI Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-AFP monoclonal antibody C3 6014

antibody
Database link: human P02771
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Fetal liver or hepatocellular carcinoma
Immunogen Alpha fetoprotein (AFP) purified from serum of a hepatoma patient
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a - Isotype Control

target relevance
Protein names Alpha-fetoprotein (Alpha-1-fetoprotein) (Alpha-fetoglobulin)
Gene names AFP,AFP HPAFP
Protein family ALB/AFP/VDB family
Mass 68678Da
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Subellular location Secreted.
Tissues Plasma. Synthesized by the fetal liver and yolk sac.
Structure Dimeric and trimeric forms have been found in addition to the monomeric form.
Post-translational modification Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.; Sulfated.
Involvement in disease DISEASE: Alpha-fetoprotein deficiency (AFPD) [MIM:615969]: A benign condition characterized by undetectable AFP levels in the amniotic fluid. Affected individuals are asymptomatic and present normal development. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Alpha-fetoprotein, hereditary persistence (HPAFP) [MIM:615970]: A benign autosomal dominant condition characterized by continued expression of alpha-fetoprotein in adult life. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P02771
The UniProt Consortium

Data

Human yolk sac tumor stained with anti-AFP polyclonal antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells..

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.