Mouse AMCase/CHIA Protein 3490

$240.00 – $800.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Tyr22-Pro473

SKU: 3490parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q91XA9
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.
molecular weight	The protein has a predicted MW of 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse AMCase/CHIA Protein 3490

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.
Protein names Acidic mammalian chitinase (AMCase) (EC 3.2.1.14) (YNL)
Gene names Chia,Chia Chia1
Protein family Glycosyl hydrolase 18 family, Chitinase class II subfamily
Mass 10090Da
Function Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. Presence in saliva and gastric juice suggests a function as a digestive enzyme.
Catalytic activity BINDING 70..71; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 97..100; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 141; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 210..213; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 360; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"
Subellular location Secreted. Cytoplasm. Cytoplasmic granule. Note=Detected in secretory granules of parotid acinar cells and gastric chief cells and secreted from them into saliva and gastric juice, respectively.
Tissues Detected in macrophages and lung epithelial cells. Detected in the acinar cells of parotid gland and von Ebner's gland but not in submandibular and sublingual glands. Detected in gastric chief cells. Also present in parotid glandular saliva and gastric juice (at protein level). Highly expressed in submandibular gland (PubMed:11085997) and stomach. Highly expressed in parotid gland but not in submandibular and sublingual glands (PubMed:12133911). In tongue, expressed only in von Ebner's gland. Expressed at lower levels in lung.
Structure Interacts with EGFR.
Target Relevance information above includes information from UniProt accession: Q91XA9
The UniProt Consortium

Data

The purity of Mouse AMCase/CHIA is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Mouse AMCase/CHIA Protein

Mouse AMCase/CHIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.