Mouse AARS1 Protein 2320

$315.00 – $1,050.00

Summary

Expression: Baculovirus-Insect Cells
Pure: Yes (SDS-PAGE)
Amino Acid Range: Met1-Asn968

SKU: 2320parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8BGQ7
express system	Baculovirus-Insect Cells
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Alanyl-tRNA synthetase 1 (AARS1) gene encodes a ubiquitously expressed class II enzyme that catalyzes the attachment of alanine to the cognate tRNA. AARS1 mutations are frequently responsible for autosomal dominant Charcot-Marie-Tooth disease type 2N (CMT2N).
molecular weight	The protein has a predicted MW of 108.43 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Mouse AARS1 Protein 2320

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Alanyl-tRNA synthetase 1 (AARS1) gene encodes a ubiquitously expressed class II enzyme that catalyzes the attachment of alanine to the cognate tRNA. AARS1 mutations are frequently responsible for autosomal dominant Charcot-Marie-Tooth disease type 2N (CMT2N).
Protein names Alanine--tRNA ligase, cytoplasmic (EC 6.1.1.7) (Alanyl-tRNA synthetase) (AlaRS) (Protein sticky) (Sti)
Gene names Aars1,Aars1 Aars
Protein family Class-II aminoacyl-tRNA synthetase family
Mass 10090Da
Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:27622773). Also edits incorrectly charged tRNA(Ala) via its editing domain (PubMed:16906134, PubMed:20010690, PubMed:25422440, PubMed:29769718).
Catalytic activity BINDING 77; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 95; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 176; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 214..216; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 216; /ligand="L-alanine"; /ligand_id="ChEBI:CHEBI:57972"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 239; /ligand="L-alanine"; /ligand_id="ChEBI:CHEBI:57972"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 243; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000250\|UniProtKB:P49588"; BINDING 605; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0000255\|HAMAP-Rule:MF_03133"; BINDING 609; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0000255\|HAMAP-Rule:MF_03133"; BINDING 723; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0000255\|HAMAP-Rule:MF_03133"; BINDING 727; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0000255\|HAMAP-Rule:MF_03133"
Subellular location Cytoplasm .
Structure Monomer (By similarity). Interacts with ANKRD16; the interaction is direct (PubMed:29769718).
Post-translational modification ISGylated.; Methylation at 'Lys-943' by METTL21C.
Domain Co
Target Relevance information above includes information from UniProt accession: Q8BGQ7
The UniProt Consortium

Data

Mouse AARS1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.