Human YKL-40/CHI3L1 Protein 3771

$210.00 – $700.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Tyr22-Thr383

SKU: 3771parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P36222
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role is not completely understood but YKL-40 is elevated in the brain and cerebrospinal fluid (CSF) in several neurological and neurodegenerative diseases associated with inflammatory processes. Yet the precise characterization of YKL-40 in dementia cases is missing. YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases. Therefore, YKL-40 quantification might have a potential for application in the evaluation of therapeutic intervention in dementias with a neuroinflammatory component.
molecular weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human YKL-40/CHI3L1 Protein 3771

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role is not completely understood but YKL-40 is elevated in the brain and cerebrospinal fluid (CSF) in several neurological and neurodegenerative diseases associated with inflammatory processes. Yet the precise characterization of YKL-40 in dementia cases is missing. YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases. Therefore, YKL-40 quantification might have a potential for application in the evaluation of therapeutic intervention in dementias with a neuroinflammatory component.
Protein names Chitinase-3-like protein 1 (39 kDa synovial protein) (Cartilage glycoprotein 39) (CGP-39) (GP-39) (hCGP-39) (YKL-40)
Gene names CHI3L1,CHI3L1
Protein family Glycosyl hydrolase 18 family
Mass 9606Da
Function Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
Catalytic activity BINDING 70..71; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 97..100; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 141; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 204..207; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"; BINDING 263; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; BINDING 352; /ligand="chitin"; /ligand_id="ChEBI:CHEBI:17029"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01258"
Subellular location Secreted, extracellular space. Cytoplasm. Cytoplasm, perinuclear region. Endoplasmic reticulum .
Tissues Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.
Structure Monomer.
Post-translational modification Glycosylated.
Target Relevance information above includes information from UniProt accession: P36222
The UniProt Consortium

Data

Immobilized Human YKL-40, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA.

The purity of Human YKL-40 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human YKL-40/CHI3L1 Protein

Human YKL-40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.