Human TSLP Protein 4541

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Tyr29-Gln159

SKU: 4541parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q969D9
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
molecular weight	The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 12 kDa&20-25 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human TSLP Protein 4541

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Protein names Thymic stromal lymphopoietin
Gene names TSLP,TSLP
Mass 9606Da
Function [Isoform 1]: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.; [Isoform 2]: May act as an antimicrobial peptide in the oral cavity and on the skin.
Subellular location Secreted .
Tissues Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva.
Structure Interacts with a receptor composed of CRLF2 and IL7R. Binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of IL7R to the high-affinity ternary complex.
Target Relevance information above includes information from UniProt accession: Q969D9
The UniProt Consortium

Data

Human TSLPR, hFc Tag captured on CM5 Chip via Protein A can bind Human TSLP, His Tag with an affinity constant of 9.21 nM as determined in SPR assay (Biacore T200).

Immobilized Human TSLP, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test).

Immobilized Human TSLP, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 0.11µg/ml determined by ELISA.

The purity of Human TSLP is greater than 95% as determined by SEC-HPLC.

Human TSLP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.