Skip to content

Human TSLP Protein 4541

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Tyr29-Gln159
SKU: 4541parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q969D9

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

molecular weight

The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 12 kDa&20-25 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TSLP Protein 4541

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Protein names
Thymic stromal lymphopoietin
Gene names
TSLP,TSLP
Mass
9606Da
Function
FUNCTION: [Isoform 1]: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. {ECO:0000269|PubMed:11418668, ECO:0000269|PubMed:11480573, ECO:0000269|PubMed:17242164}.; FUNCTION: [Isoform 2]: May act as an antimicrobial peptide in the oral cavity and on the skin. {ECO:0000269|PubMed:24850429}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11418668}.
Tissues
TISSUE SPECIFICITY: Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. {ECO:0000269|PubMed:11480573, ECO:0000269|PubMed:24850429}.
Structure
SUBUNIT: Interacts with a receptor composed of CRLF2 and IL7R. Binding of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of IL7R to the high-affinity ternary complex. {ECO:0000250|UniProtKB:Q9JIE6}.
Target Relevance information above includes information from UniProt accession : Q969D9
The UniProt Consortium

Data

SPR with Human TSLP Protein
Human TSLPR, hFc Tag captured on CM5 Chip via Protein A can bind Human TSLP, His Tag with an affinity constant of 9.21 nM as determined in SPR assay (Biacore T200).
ELISA with Human TSLP Protein
Immobilized Human TSLP, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test).
ELISA with Human TSLP Protein
Immobilized Human TSLP, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 0.11µg/ml determined by ELISA.
HPLC of Human TSLP Protein
The purity of Human TSLP is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TSLP Protein
Human TSLP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.