Human TREM1 Protein 4885

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala21-Arg200

SKU: 4885parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_061113
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear.
molecular weight	The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human TREM1 Protein 4885

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TREM1 (Triggering Receptor Expressed on Myeloid Cells 1) is a pro-inflammatory receptor expressed by phagocytes, which can also be released as a soluble molecule (sTREM1). The roles of TREM1 and sTREM1 in liver infection and inflammation are not clear.
Protein names Triggering receptor expressed on myeloid cells 1 (TREM-1) (Triggering receptor expressed on monocytes 1) (CD antigen CD354)
Mass 26387Da
Function [Isoform 1]: Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses (PubMed:10799849, PubMed:21393102). Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12 (PubMed:17568691, PubMed:25595774, PubMed:29568119). In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma (PubMed:14656437, PubMed:21659545). This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections (PubMed:17098818, PubMed:17568691). By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis (PubMed:11323674, PubMed:21393102). {ECO:0000269\|PubMed:10799849, ECO:0000269\|PubMed:11323674, ECO:0000269\|PubMed:14656437, ECO:0000269\|PubMed:17098818, ECO:0000269\|PubMed:17568691, ECO:0000269\|PubMed:21393102, ECO:0000269\|PubMed:21659545, ECO:0000269\|PubMed:25595774, ECO:0000269\|PubMed:29568119}.; [Isoform 2]: Acts as a decoy receptor, counterbalancing TREM1 pro-inflammatory activity through the neutralization of its lignad. {ECO:0000269\|PubMed:26561551}.
Subellular location [Isoform 1]: Cell membrane {ECO:0000269\|PubMed:17098818, ECO:0000269\|PubMed:21393102}; Single-pass type I membrane protein {ECO:0000305}. Note=Recruited to lipid rafts when activated. {ECO:0000269\|PubMed:17098818}.; [Isoform 2]: Secreted {ECO:0000269\|PubMed:26561551}.
Tissues Mostly expressed by immune cells of the myeloid lineage, such as monocytes, macrophages, neutrophils and dendritic cells (PubMed:10799849). Expression is associated with a mature stage of myeloid development (PubMed:11922939). Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Isoform 2 was detected in the lung, liver and mature monocytes. {ECO:0000269\|PubMed:10799849, ECO:0000269\|PubMed:11922939, ECO:0000269\|PubMed:21393102}.
Structure Monomer (PubMed:29568119). Homomultimer; when activated (PubMed:29568119). Interacts with TYROBP/DAP12 (PubMed:10799849, PubMed:15351648). Interacts with TLR4 (PubMed:17098818, PubMed:21393102). {ECO:0000269\|PubMed:10799849, ECO:0000269\|PubMed:15351648, ECO:0000269\|PubMed:17098818, ECO:0000269\|PubMed:21393102, ECO:0000269\|PubMed:29568119}.
Post-translational modification Glycosylated. {ECO:0000269\|PubMed:10799849}.
Target Relevance information above includes information from UniProt accession: Q9NP99
The UniProt Consortium

Data

Immobilized Human TREM1, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human PGLYRP1, hFc Tag with the EC50 of 0.32µg/ml determined by ELISA.

The purity of Human TREM1 is greater than 95% as determined by SEC-HPLC.

Human TREM1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.