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Human TRAIL Trimer Protein 4888

$165.00$550.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly118-Gly281
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P50591

express system

HEK293

product tag

N-His-Flag

purity

> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC

background

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.

molecular weight

The protein has a predicted MW of 60 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TRAIL Trimer Protein 4888

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily that can initiate the apoptosis pathway by binding to its associated death receptors DR4 and DR5. The activation of the TRAIL pathway in inducing tumor-selective apoptosis leads to the development of TRAIL-based cancer therapies, which include recombinant forms of TRAIL, TRAIL receptor agonists, and other therapeutic agents.
Protein names
Tumor necrosis factor ligand superfamily member 10 (Apo-2 ligand) (Apo-2L) (TNF-related apoptosis-inducing ligand) (Protein TRAIL) (CD antigen CD253)
Gene names
TNFSF10,TNFSF10 APO2L TRAIL
Protein family
Tumor necrosis factor family
Mass
32509Da
Function
FUNCTION: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed:26457518, PubMed:10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. {ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:26457518}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14609566}; Single-pass type II membrane protein {ECO:0000305}. Secreted {ECO:0000269|PubMed:14609566}. Note=Exists both as membrane-bound and soluble form. {ECO:0000269|PubMed:14609566}.
Tissues
TISSUE SPECIFICITY: Widespread; most predominant in spleen, lung and prostate.
Structure
SUBUNIT: Homotrimer (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10A mononers (PubMed:26457518). One TNFSF10 homotrimer interacts with three TNFSF10B mononers (PubMed:10549288). {ECO:0000269|PubMed:10549288, ECO:0000269|PubMed:26457518}.
Post-translational modification
PTM: Tyrosine phosphorylated by PKDCC/VLK. {ECO:0000269|PubMed:25171405}.
Domain
TOPO_DOM 1
Target Relevance information above includes information from UniProt accession : P50591
The UniProt Consortium

Data

ELISA with Human TRAIL Trimer Protein
Immobilized Human TRAIL Trimer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
HPLC of Human TRAIL Trimer Protein
The purity of Human TRAIL Trimer is greater than 90% as determined by SEC-HPLC.
SDS-PAGE gel of Human TRAIL Trimer Protein
Human TRAIL Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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