Human TPBG/5T4 Protein 2590

$1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ser32-Ser355

SKU: 2590parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q13641
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
molecular weight	The protein has a predicted MW of 62.08 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human TPBG/5T4 Protein 2590

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Protein names Trophoblast glycoprotein (5T4 oncofetal antigen) (5T4 oncofetal trophoblast glycoprotein) (5T4 oncotrophoblast glycoprotein) (M6P1) (Wnt-activated inhibitory factor 1) (WAIF1)
Gene names TPBG,TPBG 5T4
Mass 46032Da
Function FUNCTION: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. {ECO:0000269\|PubMed:22100263}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:24582434}; Single-pass type I membrane protein {ECO:0000269\|PubMed:24582434}.
Tissues TISSUE SPECIFICITY: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Post-translational modification PTM: Highly glycosylated. {ECO:0000269\|PubMed:19159218, ECO:0000269\|PubMed:24582434}.
Domain DOMAIN: The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. {ECO:0000269\|PubMed:24582434}.
Target Relevance information above includes information from UniProt accession: Q13641
The UniProt Consortium

Data

Immobilized Human TPBG, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-TPBG Antibody, hFc Tag with the EC50 of 26.8ng/ml determined by ELISA.

The purity of Human TPBG is greater than 95% as determined by SEC-HPLC.

Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.