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Human TNFSF15 Trimer Protein 4720

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Asp91-Leu251
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

O95150

express system

HEK293

product tag

N-His-Flag

purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

background

TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.

molecular weight

The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1 EU per μg by the LAL method.

Human TNFSF15 Trimer Protein 4720

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Protein names
Tumor necrosis factor ligand superfamily member 15 (TNF ligand-related molecule 1) (Vascular endothelial cell growth inhibitor) [Cleaved into: Tumor necrosis factor ligand superfamily member 15, membrane form; Tumor necrosis factor ligand superfamily member 15, secreted form]
Gene names
TNFSF15,TNFSF15 TL1 VEGI
Protein family
Tumor necrosis factor family
Mass
9606Da
Function
FUNCTION: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. {ECO:0000269|PubMed:10597252, ECO:0000269|PubMed:11911831, ECO:0000269|PubMed:11923219, ECO:0000269|PubMed:9872942}.
Subellular location
SUBCELLULAR LOCATION: Membrane {ECO:0000305|PubMed:11911831, ECO:0000305|PubMed:11923219}; Single-pass type II membrane protein {ECO:0000305|PubMed:11911831, ECO:0000305|PubMed:11923219}.; SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 15, secreted form]: Secreted.
Tissues
TISSUE SPECIFICITY: Specifically expressed in endothelial cells. Detected in monocytes, placenta, lung, liver, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine and colon. {ECO:0000269|PubMed:11911831, ECO:0000269|PubMed:11923219, ECO:0000269|PubMed:9434163, ECO:0000269|PubMed:9872942}.
Structure
SUBUNIT: Homotrimer. {ECO:0000269|PubMed:17935696, ECO:0000269|PubMed:21300286}.
Target Relevance information above includes information from UniProt accession : O95150
The UniProt Consortium

Data

SPR with Human TNFSF15 Trimer Protein
Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
ELISA with Human TNFSF15 Trimer Protein
Immobilized Human TNFSF15 Trimer, His Tag at 1 µg/ml (100 µl/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA (QC Test).
HPLC of Human TNFSF15 Trimer Protein
The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TNFSF15 Trimer Protein
Human TNFSF15 Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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