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Human TIMP-2 Protein 4679

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Cys27-Pro220
SKU: 4679parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P16035

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.

molecular weight

The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TIMP-2 Protein 4679

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
Protein names
Metalloproteinase inhibitor 2 (CSC-21K) (Tissue inhibitor of metalloproteinases 2) (TIMP-2)
Gene names
TIMP2,TIMP2
Protein family
Protease inhibitor I35 (TIMP) family
Mass
9606Da
Function
FUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. {ECO:0000269|PubMed:11710594, ECO:0000269|PubMed:2554304, ECO:0000269|PubMed:2793861}.
Catalytic activity
BINDING 27; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_note="ligand shared with metalloproteinase partner"; /evidence="ECO:0000269|PubMed:24073280, ECO:0007744|PDB:4ILW"
Subellular location
SUBCELLULAR LOCATION: Secreted.
Structure
SUBUNIT: Interacts (via the C-terminal) with MMP2 (via the C-terminal PEX domain); the interaction inhibits the MMP2 activity. {ECO:0000269|PubMed:11710594, ECO:0000269|PubMed:12032297, ECO:0000269|PubMed:1655733}.
Post-translational modification
PTM: The activity of TIMP2 is dependent on the presence of disulfide bonds.
Target Relevance information above includes information from UniProt accession : P16035
The UniProt Consortium

Data

ELISA with Human TIMP-2 Protein
Immobilized Human Human TIMP-2, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
HPLC of Human TIMP-2 Protein
The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TIMP-2 Protein
Human TIMP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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