Skip to content

Human TIGIT Protein 4405

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met22-Pro141
SKU: 4405parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q495A1

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

molecular weight

The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human TIGIT Protein 4405

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Protein names
T-cell immunoreceptor with Ig and ITIM domains (V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)
Gene names
TIGIT,TIGIT VSIG9 VSTM3
Mass
9606Da
Function
FUNCTION: Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. {ECO:0000269|PubMed:19011627}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19011627}; Single-pass type I membrane protein {ECO:0000269|PubMed:19011627}.
Tissues
TISSUE SPECIFICITY: Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). {ECO:0000269|PubMed:19011627}.
Structure
SUBUNIT: Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3. {ECO:0000269|PubMed:22421438}.
Domain
DOMAIN: Co
Target Relevance information above includes information from UniProt accession : Q495A1
The UniProt Consortium

Data

SPR with Human TIGIT Protein
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 µM as determined in SPR assay (Biacore T200).
ELISA with Human TIGIT Protein
Immobilized Human TIGIT, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA (QC Test).
ELISA with Human TIGIT Protein
Immobilized Biotinylated Human Nectin-2, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.22µg/ml determined by ELISA.
HPLC of Human TIGIT Protein
The purity of Human TIGIT is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human TIGIT Protein
Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.