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Human SMPD1 Protein 2242

$375.00$1,250.00

Summary

  • Expression: Baculovirus-Insect Cells
  • Active: Yes (catalytic)
  • Amino Acid Range: Met1-Pro628
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

NP_000534.3

express system

Baculovirus-Insect Cells

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.

molecular weight

The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human SMPD1 Protein 2242

Data

Activity assay with Human SMPD1 Protein
Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is > 1500 pmol/min/µg, as measured under the described conditions.
HPLC of Human SMPD1 Protein
The purity of Human SMPD1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human SMPD1 Protein
Human SMPD1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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