ELISA with Human Siglec-4a/MAG Protein 4569

Human Siglec-4a/MAG Protein 4569

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly20-Pro516

SKU: 4569parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P20916
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
molecular weight	The protein has a predicted MW of 81.4 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Siglec-4a/MAG Protein 4569

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2.
Protein names Myelin-associated glycoprotein (Siglec-4a)
Gene names MAG,MAG GMA
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 9606Da
Function Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity). Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane (By similarity). Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth (By similarity). Inhibits axon outgrowth by binding to RTN4R (By similarity). Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b (By similarity).
Catalytic activity BINDING 65..67; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"; BINDING 118; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"; BINDING 124..128; /ligand="a ganglioside GT1b (d18:1(4E))"; /ligand_id="ChEBI:CHEBI:78452"; /evidence="ECO:0000250\|UniProtKB:P20917"
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane raft .
Tissues Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures (PubMed:6200494, PubMed:9495552). Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths (PubMed:6200494). Detected in compact myelin (at protein level) (PubMed:6200494). Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system (PubMed:9495552).
Structure Monomer and homodimer (By similarity). Interacts (via the first three N-terminal Ig-like domains) with RTN4R and RTN4RL2 (By similarity). Interacts with RTN4R (PubMed:19052207). Interacts with isoform 2 of BSG (By similarity).
Post-translational modification N-glycosylated.; Phosphorylated on tyrosine residues.; Ubiquitinated, leading to proteasomal degradation.
Target Relevance information above includes information from UniProt accession: P20916
The UniProt Consortium

Data

Immobilized Human Siglec-4a, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-Siglec-4a Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA.

The purity of Human Siglec-4a is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Siglec-4a/MAG Protein

Human Siglec-4a on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.