Human Siglec-15/CD33L3 Protein 4463

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe20-Thr263

SKU: 4463parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q6ZMC9
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..
molecular weight	The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Siglec-15/CD33L3 Protein 4463

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates..
Protein names Sialic acid-binding Ig-like lectin 15 (Siglec-15) (CD33 antigen-like 3)
Gene names SIGLEC15,SIGLEC15 CD33L3
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 9606Da
Function Binds sialylated glycoproteins.
Catalytic activity BINDING 143; /ligand="N-acetylneuraminate"; /ligand_id="ChEBI:CHEBI:35418"; /evidence="ECO:0000250"
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.
Structure Interacts with TYROBP and HCST.
Target Relevance information above includes information from UniProt accession: Q6ZMC9
The UniProt Consortium

Data

ELISA with Human Siglec-15/CD33L3 Protein

Immobilized Human Siglec-15, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.

The purity of Human Siglec-15 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Siglec-15/CD33L3 Protein

Human Siglec-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.