ELISA with Human SG3/Secretogranin 3 Protein 3918

Human SG3/Secretogranin 3 Protein 3918

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Phe20-Leu468

SKU: 3918parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8WXD2
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.
molecular weight	The protein has a predicted MW of 52.1 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human SG3/Secretogranin 3 Protein 3918

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.
Protein names Secretogranin-3 (Secretogranin III) (SgIII)
Gene names SCG3,SCG3 UNQ2502/PRO5990
Mass 9606Da
Function Member of the granin protein family that regulates the biogenesis of secretory granules (PubMed:19357184). Acts as a sorting receptor for intragranular proteins including chromogranin A/CHGA (By similarity). May also play a role in angiogenesis. Promotes endothelial proliferation, migration and tube formation through MEK/ERK signaling pathway (PubMed:29154827).
Subellular location Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane ; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to cholesterol-enriched lipid rafts.
Tissues Detected in urine (at protein level) (PubMed:25326458). Expressed in brain, heart, kidney, liver and skeletal muscle.
Structure Interacts with CHGA (By similarity) (PubMed:19357184). Interacts with secretogranin II/SCG2 (PubMed:19357184). Interacts (via C-terminus) with CPE (By similarity).
Post-translational modification O-glycosylated.
Target Relevance information above includes information from UniProt accession: Q8WXD2
The UniProt Consortium

Data

ELISA with Human SG3/Secretogranin 3 Protein

Immobilized Human SG3, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6ng/ml determined by ELISA.

HPLC of Human SG3/Secretogranin 3 Protein

The purity of Human SG3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human SG3/Secretogranin 3 Protein

Human SG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.