Human SETD7 Protein 2467

$300.00 – $1,000.00

Summary

Expression: E.coli
Pure: Yes (HPLC)
Amino Acid Range: Met1-Lys366

SKU: 2467parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_085151
express system	E.coli
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	SETD7 is a methyltransferase that specifically catalyzes the monomethylation of lysine 4 on histone H3. A variety of studies has revealed the role of SETD7 in posttranslational modifications of non-histone proteins. Aberrant expression of SETD7 has been associated with various diseases, including cancer. As a prognostic marker of breast cancer and a novel antioxidant promoter under oxidative stress in breast cancer, SETD7 is considered a good target for the development of new epigenetic drugs.
molecular weight	The protein has a predicted MW of 41.68 kDa. The protein migrates to 47-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human SETD7 Protein 2467

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
SETD7 is a methyltransferase that specifically catalyzes the monomethylation of lysine 4 on histone H3. A variety of studies has revealed the role of SETD7 in posttranslational modifications of non-histone proteins. Aberrant expression of SETD7 has been associated with various diseases, including cancer. As a prognostic marker of breast cancer and a novel antioxidant promoter under oxidative stress in breast cancer, SETD7 is considered a good target for the development of new epigenetic drugs.
Protein names Histone-lysine N-methyltransferase SETD7 (EC 2.1.1.364) (Histone H3-K4 methyltransferase SETD7) (H3-K4-HMTase SETD7) (Lysine N-methyltransferase 7) (SET domain-containing protein 7) (SET7/9)
Protein family Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransf
Mass 40721Da
Function Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3 (PubMed:11779497, PubMed:11850410, PubMed:12540855, PubMed:12588998, PubMed:16141209). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:12540855, PubMed:12588998, PubMed:16141209). Plays a central role in the transcriptional activation of genes such as collagenase or insulin (PubMed:12588998, PubMed:16141209). Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription (PubMed:16141209). Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins (PubMed:15099517, PubMed:15525938, PubMed:16415881, PubMed:35210392). Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II (PubMed:15099517, PubMed:16415881). Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation (PubMed:15525938, PubMed:16415881, PubMed:17108971). Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS (By similarity). {ECO:0000250\|UniProtKB:Q8VHL1, ECO:0000269\|PubMed:11779497, ECO:0000269\|PubMed:11850410, ECO:0000269\|PubMed:12540855, ECO:0000269\|PubMed:12588998, ECO:0000269\|PubMed:15099517, ECO:0000269\|PubMed:15525938, ECO:0000269\|PubMed:16141209, ECO:0000269\|PubMed:16415881, ECO:0000269\|PubMed:17108971, ECO:0000269\|PubMed:35210392}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:60264, Rhea:RHEA-COMP:15543, Rhea:RHEA-COMP:15547, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; EC=2.1.1.364; Evidence={ECO:0000255\|PROSITE-ProRule:PRU00910, ECO:0000269\|PubMed:12540855}; CATALYTIC ACTIVITY: Reaction=L-lysyl-[protein] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:51736, Rhea:RHEA-COMP:9752, Rhea:RHEA-COMP:13053, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; Evidence={ECO:0000269\|PubMed:15099517, ECO:0000269\|PubMed:15525938, ECO:0000269\|PubMed:16415881}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51737; Evidence={ECO:0000269\|PubMed:15099517, ECO:0000269\|PubMed:15525938, ECO:0000269\|PubMed:16415881};
Subellular location Nucleus {ECO:0000305\|PubMed:11779497}. Chromosome {ECO:0000305\|PubMed:11850410}.
Tissues Widely expressed. Expressed in pancreatic islets. {ECO:0000269\|PubMed:16141209}.
Structure Interacts with IPF1/PDX-1. {ECO:0000269\|PubMed:12514135, ECO:0000269\|PubMed:12540855, ECO:0000269\|PubMed:16141209}.
Domain The SET domain is necessary but not sufficient for histone methyltransferase activity. {ECO:
Target Relevance information above includes information from UniProt accession: Q8WTS6
The UniProt Consortium

Data

The purity of Human SETD7 is greater than 95% as determined by SEC-HPLC.

Human SETD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.