Skip to content
Human SARS-CoV-2 IgG Lateral flow dipstick kit 7374

Human SARS-CoV-2 IgG Lateral flow dipstick kit 7374

$487.00

Summary

  • Mikrogen diagnostik lateral flow device (dipstick) for research use (RUO)
  • Human SARS-CoV-2 IgG Lateral flow dipstick kit 7374
  • Suitable for IgG detection
  • Ready-to-use
  • 20 tests
SKU: 7374 Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
target

Human SARS-CoV-2 IgG

species reactivity

Human SARS-CoV-2

applications

Lateral flow (dipstick)

assay type

Indirect & qualitative

available sizes

20 test kits

Human SARS-CoV-2 IgG Lateral flow dipstick kit 7374

kit
Assay type
Sandwich assay, lateral flow (dipstick)
Research area
Infectious Disease
Sample type
Serum, plasma, whole blood
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instructions for Use1 Each
Evaluation Form1 Each
Test Strips2 kits of [2 Vials x 10 Each]
Anti-Human IgG Conjugate500 μL
Positive Control140 μL
Negative Control140 μL
Storage
Store at 2-8°C.
Additional information
Mikrogen recomLine SARS-CoV-2 IgG [Avidity] Test

In commercial screening tests for antibody detection, the nucleocapsid protein (NP) and/or the spike protein (S) or corresponding subunits are usually used as immunodominant antigens. The Mikrogen recomLine SARS-CoV-2 IgG [avidity] line immunoassay combines these diagnostic markers and allows the identification of specific antibodies against the individual antigens. In addition, reactivities against the seasonal coronaviruses (HCoV) are detected.

The test allows the assessment of the adaptive humoral immune response to SARS-CoV-2 after infection and/or vaccination. An added benefit is that this test system determines the quality of the antibodies when avidity measurement is used.

Advantages
  • Detection of antibodies against SARS-CoV-2 after survived infection and/or vaccination.
  • Avidity determination of SARS-CoV-2 specific IgG antibodies provides additional information about the quality of the antibodies.
  • Additional information through the detection of antibodies against seasonal coronaviruses (HCoV).
  • Suitability as a screening and confirmatory test for the detection of IgG.
  • Easy and clear interpretation, featuring easy-to-read banding patterns.
  • Partial and full automation, software-based evaluation (recomScan), and integration with the laboratory information system are possible.
  • Highest sensitivity and specificity due to the use of different, recombinant SARS-CoV-2 specific antigens.
Bands
PathogenAntigenDescription
SARS-CoV-2NPNucleocapsid - antigen with the strongest immunogenicity among the coronaviruses. As a structural protein, it primarily serves to package viral genetic information and also fulfills regulatory functions.
SARS-CoV-2RBDUnits of the spike surface protein - main target antigen for neutralizing antibodies. The S1 subunits with integrated receptor binding domain (RBD) are responsible for binding to the host cell.
SARS-CoV-2S1Spike protein subunit responsible for binding to the host cell.
Seasonal coronaviruses HCoV (229E, NL63, OC43, HKU1)NPNucleocapsid - analogous to the nucleocapsid of SARS-CoV-2, found in seasonal human pathogenic ?- and ?-coronaviruses (229E, NL63 and OC43, HKU1, respectively).
target relevance
Organism
SARS-CoV-2
Structure and strains
SARS-CoV-2 consists of 4 structural proteins and 16 non-structural proteins. The 4 structural proteins are spike (S), envelope (E), nucleocapsid (N) and membrane (M) protein. The N protein is associated with the RNA genome, the S, E and M proteins together form the virus envelope. The S protein is a glycoprotein consisting of two domains, the S1 domain, which contains the receptor binding domain (RBD) responsible for the virus being able to attach to and fuse with the ACE2 receptor of the host cell, and the S2 domain including transmembrane and endodomain. On the virus surface, the S protein forms trimeric structures. N and S protein are major immunogenic proteins of the virus family Coronaviridae and are very well suited for serological detection of anti-SARS-CoV-2 antibodies. Recombinant versions of both proteins are already widely used in different test systems.
Disease
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a strain of coronavirus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. The virus previously had the provisional name 2019 novel coronavirus (2019-nCoV),and has also been called human coronavirus 2019 (HCoV-19 or hCoV-19). SARS-CoV-2 is a positive-sense single-stranded RNA virus that is contagious in humans.
Detection and diagnosis
Neutralizing antibodies during host immune responses are predominantly directed against S protein, which is supposedly suitable for very specific test settings as it is less conserved within the family of Coronaviridae than the other immunogenic antigens.However, recent studies have shown that antibodies against N protein are detectable earlier in mild infections than antibodies directed against S protein. As an acute marker in particular, it can be beneficial to use N protein for most sensitive early detection. In the further course of the immune response, the N protein antibody level decreases, whereas antibodies against S protein are detectable for a longer time. Various studies show that anti-SARS-CoV-2 antibodies of the IgA and IgM class are detectable in the median at the earliest from about six days after the onset of symptoms, increase in the further course of the disease and decrease again between 18 and 35 days. Antibodies of the IgG class are detectable in the median ten to eighteen days after onset of symptoms and are detectable over several months.

Publications

pmidtitleauthorscitation
We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
#N/A
#
Please enter your product and batch number here to retrieve product datasheet, SDS, and QC information.

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Related Products