Human PTP alpha/PTPRA Protein 2226

$345.00 – $1,150.00

Summary

Expression: Baculovirus-Insect Cells
Pure: Yes (SDS-PAGE)
Amino Acid Range: Ala183-Lys802

SKU: 2226parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P18433
express system	Baculovirus-Insect Cells
product tag	C-His
purity	> 90% as determined by Tris-Bis PAGE
background	The receptor protein-tyrosine phosphatase α (encoded by PTPRA), a 130-kDa transmembrane PTP, has shown to be a physiological regulator for PTK c-Src and the kinases involved in integrin signaling pathway including FAK and P130cas. The activation of PTPRA on Src Y530 dephosphorylation led to cellular transformation, which suggested that PTPRA might be an oncogenic gene.
molecular weight	The protein has a predicted MW of 73.00 kDa. The protein migrates to 68-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human PTP alpha/PTPRA Protein 2226

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The receptor protein-tyrosine phosphatase α (encoded by PTPRA), a 130-kDa transmembrane PTP, has shown to be a physiological regulator for PTK c-Src and the kinases involved in integrin signaling pathway including FAK and P130cas. The activation of PTPRA on Src Y530 dephosphorylation led to cellular transformation, which suggested that PTPRA might be an oncogenic gene.
Protein names Receptor-type tyrosine-protein phosphatase alpha (Protein-tyrosine phosphatase alpha) (R-PTP-alpha) (EC 3.1.3.48)
Gene names PTPRA,PTPRA PTPA PTPRL2
Protein family Protein-tyrosine phosphatase family, Receptor class 4 subfamily
Mass 9606Da
Function Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation (By similarity). Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42 (By similarity).
Catalytic activity #N/A
Subellular location Cell membrane ; Single-pass type I membrane protein. Cell junction, focal adhesion. Note=Localizes to focal adhesion sites following integrin engagement.
Structure Part of a complex comprised of PTPRA, BCAR1, BCAR3 (via SH2 domain), and SRC (PubMed:22801373). Within the complex, interacts (when phosphorylated on Tyr-798) with BCAR3 (via SH2 domain) (PubMed:22801373). Interacts with GRB2 (PubMed:22801373).
Post-translational modification Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions.
Target Relevance information above includes information from UniProt accession: P18433
The UniProt Consortium

Data

SDS-PAGE gel of Human PTP alpha/PTPRA Protein

Human PTP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.