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Human PSMA/FOLH1 Protein 5183

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Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Lys44-Ala750
SKU: 5183parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q04609

express system

HEK293

product tag

N-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

molecular weight

The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human PSMA/FOLH1 Protein 5183

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Protein names
Glutamate carboxypeptidase 2 (EC 3.4.17.21) (Cell growth-inhibiting gene 27 protein) (Folate hydrolase 1) (Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Glutamate carboxypeptidase II) (GCPII) (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Prostate-specific membrane antige
Gene names
FOLH1,FOLH1 FOLH NAALAD1 PSM PSMA GIG27
Protein family
Peptidase M28 family, M28B subfamily
Mass
9606Da
Function
FUNCTION: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.; FUNCTION: Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9809977}; Single-pass type II membrane protein {ECO:0000269|PubMed:9809977}.; SUBCELLULAR LOCATION: [Isoform PSMA']: Cytoplasm {ECO:0000269|PubMed:9809977}.
Tissues
TISSUE SPECIFICITY: Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-9 is specifically expressed in prostate cancer. {ECO:0000269|PubMed:11092759, ECO:0000269|PubMed:14716746, ECO:0000269|PubMed:16555021, ECO:0000269|PubMed:17150306}.
Structure
SUBUNIT: Homodimer. {ECO:0000269|PubMed:16467855, ECO:0000269|PubMed:19301871}.
Post-translational modification
PTM: The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.; PTM: The N-terminus is blocked.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : Q04609
The UniProt Consortium

Data

ELISA with Human PSMA/FOLH1 Protein
Immobilized Human PSMA, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
HPLC of Human PSMA/FOLH1 Protein
The purity of Human PSMA is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human PSMA/FOLH1 Protein
Human PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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