Human Periostin/OSF-2 Protein 3560

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asn22-Gln836

SKU: 3560parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q15063
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
molecular weight	The protein has a predicted MW of 92.1 kDa. Due to glycosylation, the protein migrates to 93-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Periostin/OSF-2 Protein 3560

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Periostin is a matricellular protein that is expressed in several tissues during embryonic development; however, its expression in adults is mostly restricted to collagen-rich connective tissues. Periostin is expressed only briefly during kidney development, but it is not normally detected in the adult kidney. Recent evidence has revealed that periostin is aberrantly expressed in several forms of chronic kidney disease (CKD), and that its expression correlates with the degree of interstitial fibrosis and the decline in renal function.
Protein names Periostin (PN) (Osteoblast-specific factor 2) (OSF-2)
Gene names POSTN,POSTN OSF2
Mass 9606Da
Function Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
Subellular location Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix. Note=Colocalizes with BMP1 in the Golgi.
Tissues Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Expressed in the kidney (PubMed:21763681). Expressed in the lung (PubMed:22079858). Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
Structure Homodimer (PubMed:29754429). Interacts with BMP1 and fibronectin.
Post-translational modification Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).
Target Relevance information above includes information from UniProt accession: Q15063
The UniProt Consortium

Data

ELISA with Human Periostin/OSF-2 Protein

Immobilized Human Periostin, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Periostin Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

The purity of Human Periostin is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Periostin/OSF-2 Protein

Human Periostin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.