Human NRAS Protein 2432

$300.00 – $1,000.00

Summary

Expression: E.coli
Pure: Yes (HPLC)
Amino Acid Range: Met1-Cys186

SKU: 2432parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P01111
express system	E.coli
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM.
molecular weight	The protein has a predicted MW of 22.29 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human NRAS Protein 2432

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM.
Protein names GTPase NRas (EC 3.6.5.2) (Transforming protein N-Ras)
Gene names NRAS,NRAS HRAS1
Protein family Small GTPase superfamily, Ras family
Mass 9606Da
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Catalytic activity BINDING 10..18; /ligand="GTP"; /ligand_id="ChEBI:CHEBI:37565"; /evidence="ECO:0000269\|Ref.25"; BINDING 29..30; /ligand="GTP"; /ligand_id="ChEBI:CHEBI:37565"; /evidence="ECO:0000269\|Ref.25"; BINDING 57..61; /ligand="GTP"; /ligand_id="ChEBI:CHEBI:37565"; /evidence="ECO:0000255"; BINDING 116..119; /ligand="GTP"; /ligand_id="ChEBI:CHEBI:37565"; /evidence="ECO:0000269\|Ref.25"
Subellular location Cell membrane ; Lipid-anchor ; Cytoplasmic side. Golgi apparatus membrane ; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.
Structure Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts (active GTP-bound form) with RASSF7 (PubMed:21278800).
Post-translational modification Palmitoylated by the ZDHHC9-GOLGA7 complex (PubMed:16000296). Depalmitoylated by ABHD17A, ABHD17B and ABHD17C (PubMed:26701913). A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi (PubMed:15705808, PubMed:16000296, PubMed:2661017, PubMed:26701913).; Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).; Fatty-acylated at Lys-169 and/or Lys-170.; Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.; Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.; (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.
Target Relevance information above includes information from UniProt accession: P01111
The UniProt Consortium

Data

The purity of Human NRAS is greater than 90% as determined by SEC-HPLC.

Human NRAS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.