Cell based assay with Human Noggin Protein 4688

Human Noggin Protein 4688

$270.00 – $900.00

Summary

Expression: CHO
Active: Yes (cell based assay)
Amino Acid Range: Gln28-Cys232

SKU: 4688parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q13253
express system	CHO
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE
background	Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.
molecular weight	The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.05EU per μg by the LAL method.

Human Noggin Protein 4688

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β-catenin, EGFR, ERK and Akt.
Protein names Noggin
Gene names NOG,NOG
Protein family Noggin family
Mass 9606Da
Function Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732).
Subellular location Secreted.
Structure Homodimer. Interacts with GDF5; inhibits chondrocyte differentiation.
Target Relevance information above includes information from UniProt accession: Q13253
The UniProt Consortium

Data

Cell based assay with Human Noggin Protein

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP-4.

The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.

Human Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.