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Human Noggin Protein 4688

$270.00$900.00

Summary

  • Expression: CHO
  • Active: Yes (cell based assay)
  • Amino Acid Range: Gln28-Cys232
SKU: 4688parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q13253

express system

CHO

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE

background

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

molecular weight

The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.05EU per μg by the LAL method.

Human Noggin Protein 4688

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.
Protein names
Noggin
Gene names
NOG,NOG
Protein family
Noggin family
Mass
9606Da
Function
FUNCTION: Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732). {ECO:0000269|PubMed:12478285, ECO:0000269|PubMed:21976273, ECO:0000269|PubMed:26643732}.
Subellular location
SUBCELLULAR LOCATION: Secreted.
Structure
SUBUNIT: Homodimer. Interacts with GDF5; inhibits chondrocyte differentiation. {ECO:0000269|PubMed:12478285, ECO:0000269|PubMed:21976273, ECO:0000269|PubMed:24098149}.
Target Relevance information above includes information from UniProt accession : Q13253
The UniProt Consortium

Data

Cell based assay with Human Noggin Protein
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP-4.
HPLC of Human Noggin Protein
The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Noggin Protein
Human Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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