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Human NKG2A&CD94 Protein 4822

$330.00$1,100.00

Summary

  • Expression: HEK293
  • Functional: Yes (binding assay)
  • Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
SKU: 4822parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P26715(NKG2A)&&Q13241(CD94)

express system

HEK293

product tag

C-mFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

molecular weight

The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human NKG2A&CD94 Protein 4822

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with Human NKG2A&CD94 Protein
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
ELISA with Human NKG2A&CD94 Protein
Immobilized Human NKG2A&CD94, mFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Binding assay with Human NKG2A&CD94 Protein
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15µg/ml.
HPLC of Human NKG2A&CD94 Protein
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human NKG2A&CD94 Protein
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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