Human Nectin-4 Protein 4608

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly32-Ser349

SKU: 4608parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q96NY8
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
molecular weight	The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Nectin-4 Protein 4608

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Protein names Nectin-4 (Ig superfamily receptor LNIR) (Nectin cell adhesion molecule 4) (Poliovirus receptor-related protein 4) [Cleaved into: Processed poliovirus receptor-related protein 4]
Gene names NECTIN4,NECTIN4 LNIR PRR4 PVRL4
Protein family Nectin family
Mass 9606Da
Function Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; (Microbial infection) Acts as a receptor for measles virus.
Subellular location Cell membrane ; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
Tissues Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
Structure Self-associates. Interacts via its Ig-like V-type domain with NECTIN1 Ig-like V-type domain. Interacts via its C-terminus with AFDN.; (Microbial infection) Interacts with measles virus Hemagglutinin protein (PubMed:22048310, PubMed:23202587).
Post-translational modification The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.
Target Relevance information above includes information from UniProt accession: Q96NY8
The UniProt Consortium

Data

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).

Immobilized Human Nectin-4, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1µg/ml determined by ELISA (QC Test).

The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

Human Nectin-4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.