no metadatahttps://benchmarkantibodies.com/product/human-nectin-4-protein-4534/
Skip to content

Human Nectin-4 Protein 4534

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly32-Val351
SKU: 4534parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q96NY8

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

molecular weight

The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Nectin-4 Protein 4534

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Protein names
Nectin-4 (Ig superfamily receptor LNIR) (Nectin cell adhesion molecule 4) (Poliovirus receptor-related protein 4) [Cleaved into: Processed poliovirus receptor-related protein 4]
Gene names
NECTIN4,NECTIN4 LNIR PRR4 PVRL4
Protein family
Nectin family
Mass
9606Da
Function
FUNCTION: Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; FUNCTION: (Microbial infection) Acts as a receptor for measles virus. {ECO:0000269|PubMed:22048310, ECO:0000269|PubMed:23202587}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}. Cell junction, adherens junction {ECO:0000269|PubMed:11544254}. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; SUBCELLULAR LOCATION: [Processed poliovirus receptor-related protein 4]: Secreted {ECO:0000269|PubMed:15784625}. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
Tissues
TISSUE SPECIFICITY: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. {ECO:0000269|PubMed:11544254, ECO:0000269|PubMed:17474988}.
Structure
SUBUNIT: Self-associates. Interacts via its Ig-like V-type domain with NECTIN1 Ig-like V-type domain. Interacts via its C-terminus with AFDN. {ECO:0000269|PubMed:11544254, ECO:0000269|PubMed:12011057, ECO:0000269|PubMed:22902367}.; SUBUNIT: (Microbial infection) Interacts with measles virus Hemagglutinin protein (PubMed:22048310, PubMed:23202587). {ECO:0000269|PubMed:22048310, ECO:0000269|PubMed:23202587}.
Post-translational modification
PTM: The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. {ECO:0000269|PubMed:15784625}.
Target Relevance information above includes information from UniProt accession : Q96NY8
The UniProt Consortium

Data

ELISA with Human Nectin-4 Protein
Immobilized Human Nectin-4 at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
ELISA with Human Nectin-4 Protein
Immobilized Human Nectin-4, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27µg/ml determined by ELISA.
HPLC of Human Nectin-4 Protein
The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Nectin-4 Protein
Human Nectin-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.