Skip to content

Human Nectin-2/CD112 Protein 4590

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln32-Leu360
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q92692

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

molecular weight

The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Nectin-2/CD112 Protein 4590

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Protein names
Nectin-2 (Herpes virus entry mediator B) (Herpesvirus entry mediator B) (HveB) (Nectin cell adhesion molecule 2) (Poliovirus receptor-related protein 2) (CD antigen CD112)
Gene names
NECTIN2,NECTIN2 HVEB PRR2 PVRL2
Protein family
Nectin family
Mass
9606Da
Function
FUNCTION: Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705). Probable cell adhesion protein (PubMed:9657005). {ECO:0000269|PubMed:26755705, ECO:0000269|PubMed:9657005}.; FUNCTION: (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV). {ECO:0000269|PubMed:11602758, ECO:0000269|PubMed:9657005}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
Tissues
TISSUE SPECIFICITY: Ubiquitous.
Structure
SUBUNIT: Can form trans-heterodimers with NECTIN3 (By similarity). Interacts with CD226 or with PVRIG; these interactions are competitive and have a differential functional outcome on T-cell activation, either positive or negative, respectively. Binds with low affinity to TIGIT. {ECO:0000250, ECO:0000269|PubMed:15607800, ECO:0000269|PubMed:19011627, ECO:0000269|PubMed:22927415}.; SUBUNIT: (Microbial infection) Interacts with herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV) envelope glycoprotein D (PubMed:11602758, PubMed:9657005). {ECO:0000269|PubMed:11602758, ECO:0000269|PubMed:9657005}.
Target Relevance information above includes information from UniProt accession : Q92692
The UniProt Consortium

Data

SPR with Human Nectin-2/CD112 Protein
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 µM as determined in SPR assay (Biacore T200).
SPR with Human Nectin-2/CD112 Protein
Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 µM as determined in SPR assay (Biacore T200).
ELISA with Human Nectin-2/CD112 Protein
Immobilized Human Nectin-2, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17µg/ml determined by ELISA (QC Test).
HPLC of Human Nectin-2/CD112 Protein
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Nectin-2/CD112 Protein
Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.