| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| target | Human/Mouse/Rat GDF-8 |
| accession | O14793 |
| express system | HEK293 |
| product tag | No Tag |
| purity | > 95% as determined by Tris-Bis PAGE |
| activity | Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail. |
| molecular weight | The protein has a predicted MW of 12.40 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Tris-Bis PAGE result. |
| available size | 100 µg, 500 µg |
| endotoxin | Less than 1EU per ug by the LAL method. |
Human/Mouse/Rat GDF-8 Protein 2000
$405.00 – $1,300.00
Summary
- Expression System: HEK293
- Product Tag: No Tag
- Purity: > 95% as determined by Tris-Bis PAGE
- Activity: Immobilized Human/Mouse/Rat GDF-8, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Activin RIIB, mFc Tag with the EC50 of 20.8ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Asp267-Ser375
Human/Mouse/Rat GDF-8 Protein 2000
| protein |
|---|
| Size and concentration 100,500µg and |
| Form Liquid |
| Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Storage buffer Shipped with dry ice. |
| Purity > 95% as determined by Tris-Bis PAGE |
| target relevance |
|---|
| Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain. |
| Protein names Growth/differentiation factor 8 (GDF-8) (Myostatin) |
| Gene names MSTN,MSTN GDF8 |
| Protein family TGF-beta family |
| Mass 9606Da |
| Function FUNCTION: Acts specifically as a negative regulator of skeletal muscle growth. {ECO:0000250|UniProtKB:O08689}. |
| Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O08689}. |
| Structure SUBUNIT: Homodimer; disulfide-linked (PubMed:27625211). Interacts with WFIKKN2, leading to inhibit its activity (PubMed:12595574). Interacts with FST3 (PubMed:17878677). {ECO:0000269|PubMed:12595574, ECO:0000269|PubMed:17878677, ECO:0000269|PubMed:27625211}. |
| Post-translational modification PTM: Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase. {ECO:0000250|UniProtKB:O08689}. |
| Target Relevance information above includes information from UniProt accession : O14793 |
| The UniProt Consortium |
Data
 |
| Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected-ED50-for this effect is < 30 ng/ml. |
 |
| Human/Mouse/Rat GDF-8 on Tris-Bis PAGE under reduced (R) condition. The purity is greater than 95%. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
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