Human MICA alpha 3 Protein 2734

$375.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg105-Ser200

SKU: 2734parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	NP_001276081
express system	HEK293
product tag	N-mFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
molecular weight	The protein has a predicted MW of 36.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human MICA alpha 3 Protein 2734

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..
Protein names MHC class I polypeptide-related sequence A (MIC-A)
Protein family MHC class I family, MIC subfamily
Mass 42915Da
Function Widely expressed membrane-bound protein which acts as a ligand to stimulate an activating receptor KLRK1/NKG2D, expressed on the surface of essentially all human natural killer (NK), gammadelta T and CD8 alphabeta T-cells (PubMed:11777960, PubMed:11491531). Up-regulated in stressed conditions, such as viral and bacterial infections or DNA damage response, serves as signal of cellular stress, and engagement of KLRK1/NKG2D by MICA triggers NK-cells resulting in a range of immune effector functions, such as cytotoxicity and cytokine production (PubMed:10426993). {ECO:0000269\|PubMed:10426993, ECO:0000269\|PubMed:11224526, ECO:0000269\|PubMed:11491531, ECO:0000269\|PubMed:11777960, ECO:0000269\|PubMed:9497295}.
Subellular location Cell membrane {ECO:0000269\|PubMed:10363723, ECO:0000269\|PubMed:18287244, ECO:0000269\|PubMed:21928280, ECO:0000269\|PubMed:8901601}; Single-pass type I membrane protein {ECO:0000269\|PubMed:10363723, ECO:0000269\|PubMed:18287244, ECO:0000269\|PubMed:8901601}. Cytoplasm {ECO:0000269\|PubMed:10363723, ECO:0000269\|PubMed:18287244, ECO:0000269\|PubMed:8901601}. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. {ECO:0000269\|PubMed:10363723, ECO:0000269\|PubMed:18287244, ECO:0000269\|PubMed:8901601}.
Tissues Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T-cells. {ECO:0000269\|PubMed:10359807, ECO:0000269\|PubMed:10363723, ECO:0000269\|PubMed:10691930, ECO:0000269\|PubMed:12902493, ECO:0000269\|PubMed:17565371, ECO:0000269\|PubMed:8901601, ECO:0000269\|PubMed:9396860}.
Structure Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICA while HCST acts as an adapter molecule which enables signal transduction. Interacts with PDIA6 on the surface of tumor cells, leading to disulfide bond reduction which is required for release of MICA from tumor cells. {ECO:0000269\|PubMed:10426993, ECO:0000269\|PubMed:10426994, ECO:0000269\|PubMed:11323699, ECO:0000269\|PubMed:11491531, ECO:0000269\|PubMed:17495932, ECO:0000269\|PubMed:8901601, ECO:0000269\|PubMed:9396860}.; (Microbial infection) Interacts with human cytomegalovirus/HHV-5 protein UL142. {ECO:0000269\|PubMed:19793804}.
Post-translational modification N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. {ECO:0000269\|PubMed:8901601}.; Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. {ECO:0000269\|PubMed:12370336, ECO:0000269\|PubMed:12384702}.; Palmitoylated on cysteine residues in the cytoplasmic tail leading to its association with membrane microdomains enriched in cholesterol. {ECO:0000269\|PubMed:21928280}.; N-glycosylation is necessary for cell surface expression. {ECO:0000269\|PubMed:24872415}.; (Microbial infection) Ubiquitinated by human herpesvirus 8 protein K5, leading to degradation. {ECO:0000269\|PubMed:18230726}.
Target Relevance information above includes information from UniProt accession: Q29983
The UniProt Consortium

Data

Immobilized Human MICA alpha 3, mFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.10µg/ml determined by ELISA.

The purity of Human MICA alpha 3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human MICA alpha 3 Protein

Human MICA alpha 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.