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Human LRRC15/LIB Protein 4739

$315.00$1,050.00

Summary

  • Expression: CHO
  • Functional: Yes (ELISA)
  • Amino Acid Range: Tyr22-Gly538
SKU: 4739parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q8TF66

express system

CHO

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.  LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

molecular weight

The protein has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human LRRC15/LIB Protein 4739

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.  LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Protein names
Leucine-rich repeat-containing protein 15 (Leucine-rich repeat protein induced by beta-amyloid homolog) (hLib)
Gene names
LRRC15,LRRC15 LIB
Mass
9606Da
Function
FUNCTION: (Microbial infection) Modulates the ability of SARS-CoV-2 to infect host cells through interaction with the spike protein (PubMed:36228039, PubMed:36735681, PubMed:36757924). Does not act as a SARS-CoV-2 entry receptor but sequesters virions and antagonizes in trans SARS-CoV-2 infection of ACE2(+) cells when expressed on nearby cells (PubMed:36228039, PubMed:36757924). {ECO:0000269|PubMed:36228039, ECO:0000269|PubMed:36735681, ECO:0000269|PubMed:36757924}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:36228039, ECO:0000269|PubMed:36735681, ECO:0000269|PubMed:36757924}; Single-pass type I membrane protein {ECO:0000255}.
Tissues
TISSUE SPECIFICITY: Expressed in brain and placenta (PubMed:11785964). Expressed in lung fibroblasts (PubMed:36228039, PubMed:36757924). Expressed in chodrocytes (PubMed:34702854). {ECO:0000269|PubMed:11785964, ECO:0000269|PubMed:34702854, ECO:0000269|PubMed:36228039, ECO:0000269|PubMed:36757924}.
Structure
SUBUNIT: (Microbial infection) Interacts with human coronavirus SARS-CoV-2 spike protein (via RBD domain); the interaction is direct and sequesters virions at the cell surface. {ECO:0000269|PubMed:36228039, ECO:0000269|PubMed:36735681, ECO:0000269|PubMed:36757924}.; SUBUNIT: [Isoform 1]: (Microbial infection) Interacts with human coronavirus SARS-CoV-2 spike protein (via RBD domain); the interaction is direct. {ECO:0000269|PubMed:36757924}.; SUBUNIT: [Isoform 2]: (Microbial infection) Interacts with human coronavirus SARS-CoV-2 spike protein (via RBD domain); the interaction is direct. {ECO:0000269|PubMed:36757924}.
Target Relevance information above includes information from UniProt accession : Q8TF66
The UniProt Consortium

Data

ELISA with Human LRRC15/LIB Protein
Immobilized Human LRRC15, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
HPLC of Human LRRC15/LIB Protein
The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human LRRC15/LIB Protein
Human LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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