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Human LIF Protein 4392

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ser23-Phe202
SKU: 4392parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P15018

express system

HEK293

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

molecular weight

The protein has a predicted MW of 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.1EU per μg by the LAL method.

Human LIF Protein 4392

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.
Protein names
Leukemia inhibitory factor (LIF) (Differentiation-stimulating factor) (D factor) (Melanoma-derived LPL inhibitor) (MLPLI) (Emfilermin)
Gene names
LIF,LIF HILDA
Protein family
LIF/OSM family
Mass
9606Da
Function
FUNCTION: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Subellular location
SUBCELLULAR LOCATION: Secreted.
Target Relevance information above includes information from UniProt accession : P15018
The UniProt Consortium

Data

ELISA with Human LIF Protein
Immobilized Human LIF at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA.
Cell based assay with Human LIF Protein
Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50 for this effect is 1-5 ng/mL.
HPLC of Human LIF Protein
The purity of Human LIF is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human LIF Protein
Human LIF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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