SPR with Human IL-7R alpha/CD127 Protein 4894

Human IL-7R alpha/CD127 Protein 4894

$315.00 – $1,050.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Glu21-Gly236

SKU: 4894parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P16871
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
molecular weight	The protein has a predicted MW of 25.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-7R alpha/CD127 Protein 4894

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth.
Protein names Interleukin-7 receptor subunit alpha (IL-7 receptor subunit alpha) (IL-7R subunit alpha) (IL-7R-alpha) (IL-7RA) (CDw127) (CD antigen CD127)
Protein family Type I cytokine receptor family, Type 4 subfamily
Mass 51579Da
Function Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein {ECO:0000269\|PubMed:31748347}.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Secreted.
Structure The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R. Interacts with CD53 (PubMed:31748347). {ECO:0000269\|PubMed:19141282, ECO:0000269\|PubMed:31748347}.
Post-translational modification N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. {ECO:0000269\|PubMed:19141282}.
Domain The WSXWS motif appears to be necessary for proper protein folding and thereby efficient int
Target Relevance information above includes information from UniProt accession: P16871
The UniProt Consortium

Data

SPR with Human IL-7R alpha/CD127 Protein

Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200).

The purity of Human IL-7R alpha is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IL-7R alpha/CD127 Protein

Human IL-7R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.