Cell based assay with Human IL-7 Protein 4751

Human IL-7 Protein 4751

$210.00 – $700.00

Summary

Expression: E.coli
Active: Yes (cell based assay)
Amino Acid Range: Asp26-His177

SKU: 4751parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P13232
express system	E.coli
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
molecular weight	The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.1EU per μg by the LAL method.

Human IL-7 Protein 4751

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
Protein names Interleukin-7 (IL-7)
Gene names IL7,IL7
Protein family IL-7/IL-9 family
Mass 9606Da
Function Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed:25870237, PubMed:7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed:8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed:18523275, PubMed:20974963).
Subellular location Secreted .
Structure Interacts with IL7R and CSF2RG.
Target Relevance information above includes information from UniProt accession: P13232
The UniProt Consortium

Data

Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-7 at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 52.5ng/ml determined by ELISA.

Cell based assay with Human IL-7 Protein

Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC tested).

The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.

Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.