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Human IL-3 Protein 4549

$240.00$800.00

Summary

  • Expression: E.coli
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ala20-Phe152
SKU: 4549parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P08700

express system

E.coli

product tag

N-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

molecular weight

The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-3 Protein 4549

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
Protein names
Interleukin-3 (IL-3) (Hematopoietic growth factor) (Mast cell growth factor) (MCGF) (Multipotential colony-stimulating factor) (P-cell-stimulating factor)
Gene names
IL3,IL3
Protein family
IL-3 family
Mass
9606Da
Function
FUNCTION: Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed:2556442). Stimulates also mature basophils, eosinophils, and monocytes to become functionally activated (PubMed:10779277, PubMed:32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed:23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed:12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed:29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed:27862234). {ECO:0000250|UniProtKB:P01586, ECO:0000269|PubMed:10779277, ECO:0000269|PubMed:12816992, ECO:0000269|PubMed:23226269, ECO:0000269|PubMed:2556442, ECO:0000269|PubMed:27862234, ECO:0000269|PubMed:29374162, ECO:0000269|PubMed:32889153}.
Subellular location
SUBCELLULAR LOCATION: Secreted.
Tissues
TISSUE SPECIFICITY: Activated T-cells, mast cells, natural killer cells.
Structure
SUBUNIT: Interacts with IL3RA (PubMed:29374162). {ECO:0000269|PubMed:29374162}.
Target Relevance information above includes information from UniProt accession : P08700
The UniProt Consortium

Data

ELISA with Human IL-3 Protein
Immobilized Human IL-3, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 0.46µg/ml determined by ELISA (QC Test).
Cell based assay with Human IL-3 Protein
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is < 0.2 ng/mL.
HPLC of Human IL-3 Protein
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-3 Protein
Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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