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Human IL-23R Protein 4519

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly24-Gly355
SKU: 4519parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q5VWK5

express system

HEK293

product tag

C-mFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade.

molecular weight

The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human IL-23R Protein 4519

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade.
Protein names
Interleukin-23 receptor (IL-23 receptor) (IL-23R)
Gene names
IL23R,IL23R
Protein family
Type I cytokine receptor family, Type 2 subfamily
Mass
9606Da
Function
FUNCTION: Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. {ECO:0000269|PubMed:12023369}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12023369}; Single-pass type I membrane protein {ECO:0000269|PubMed:12023369}.
Tissues
TISSUE SPECIFICITY: Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells. {ECO:0000269|PubMed:12023369, ECO:0000269|PubMed:16372191}.
Structure
SUBUNIT: Heterodimer with IL12RB1. In presence of IL23, the heterodimer forms the IL23 receptor. Interacts with JAK2 and in presence of IL23 with STAT3. {ECO:0000269|PubMed:12023369}.
Post-translational modification
PTM: Phosphorylated in response to IL23. {ECO:0000269|PubMed:12023369}.
Target Relevance information above includes information from UniProt accession : Q5VWK5
The UniProt Consortium

Data

SPR with Human IL-23R Protein
Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His-Avi Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200).
ELISA with Human IL-23R Protein
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).
HPLC of Human IL-23R Protein
The purity of Human IL-23R is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-23R Protein
Human IL-23R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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